Should I lock my suitcase/instrument case for the flight?

No.

Many suitcases and ALL instruments are opened up by security at the airport.  If the case is locked, and you are not using a TSA approved lock, they will break the lock, and possibly your suitcase/instrument case.

If you are locking it because you are afraid it might open, use a luggage strap instead.

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Posted in: Luggage - Carry On, Traveling with Instruments